Immunologic Techniques, Microbial Control and Therapeutics

Immunological techniques include both experimental methods to study the immune system and methods to generate or use immunological reagents as experimental tools. The most common immunological methods relate to the production and use of antibodies to detect specific proteins in biological samples. Various laboratory techniques exist that rely on the use of antibodies to visualize components of microorganisms or other cell types and to distinguish one cell or organism type from another. Immunologic techniques are used for: Quantitating and detecting antibodies and/or antigens, Purifying immunoglobulin’s, lymphokines and other molecules of the immune system, Isolating antigens and other substances important in immunological processes, Labelling antigens and antibodies, Localizing antigens and/or antibodies in tissues and cells, Detecting, and fractionating immunocompetent cells, Assaying for cellular immunity, Documenting cell-cell interactions, Initiating immunity and unresponsiveness, Transplanting tissues, Studying items closely related to immunity such as complement, reticuloendothelial system and others, Molecular techniques for studying immune cells and their receptors, Imaging of the immune system, Methods for production or their fragments in eukaryotic and prokaryotic cells.

 

  • Antibody generation
  • Immunoblotting
  • ELISA

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